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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis - Urine formation and composition |
| Immunology and Serology | 5-10% | - Immune system principles and components - Autoimmune and immunodeficiency disorders - Infectious disease serology - Immunologic testing methodologies |
| Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests - Endocrinology and therapeutic drug monitoring |
| Blood Banking (Immunohematology) | 15-20% | - Blood group systems and antigens - Blood components and storage - Compatibility testing - Antibody detection and identification - Transfusion reactions and adverse events |
| Microbiology | 15-20% | - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Mycology, parasitology, and virology |
| Laboratory Operations | 5-10% | - Laboratory safety and regulatory compliance - Quality control and quality assurance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes |
| Hematology | 20-25% | - Hemostasis and coagulation testing - White blood cell morphology and diseases - Red blood cell physiology and disorders - Routine and automated hematology procedures |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
A slow, progressive drift of values away from the mean is called a trend. Trends occur when a particular analyte value slowly and progressively changes in one direction from one run to the next. Monitoring data for trends is part of good laboratory practice and is one indicator of many that helps laboratorians determine whether the measurement system is fucntioning appropriately.
A slow, progressive drift of values away from the mean is called a:
A. Sh
B. Dispersion
C. Trend
D. Coefficient of Deviation
E. Variance
Question 2
Bone marrow biopsies are helpful diagnostic tools in:
Evaluation of anemia and thrombocytopenia
Evaluation of cytopenias
Diagnosing leukemias
Can be part of evaluation for FUO
Bone marrow biopsies are NOT used as screens for hematologic issues during routine check-ups.
Hematology
In which of the following situations would a bone marrow aspirate and biopsy possibly be used as an aid for diagnosis? (Select all that apply)
A. Evaluation of anemia and thrombocytopenia
B. Screening for hematologic issues during routine check-ups
C. Diagnosis of leukemias
D. Evaluation of fever of unknown origin (FUO)
E. Evaluation of cytopenias
Question 3
Incompatiblity involving the ABO blood group system can cause the most severe type of transfusion reaction.
The cause of the most severe life-threatening hemolytic transfusion reactions is:
A. Anti-M
B. Anti-D
C. Anti-Fya
D. Anti-A, Anti-B, Anti-A, B
Question 4
P. vivax characteristically displays Schuffner's dots and often enlarged RBCs along with brownish granules. P.
vivax can also have 12-24 merozoies in each cell, actually filling the entire RBC. This parasite also has very irreglar shapes often referred to as "Ameboid".
P. falciparum and P. malariae do not display Shuffner's dots, therefore could not be the correct choice.
P. ovale does display Shuffner's dots in all stages, but characteristically has about 8-12 merozoites in rosettes or irregular clusters inside the RBC. Also, P. ovale characteristically shows enlarged, ovoid RBCs with fimbriated edges.
Identify the parasite of a patient with suspected malaria who demonstrates the following findings on a blood smear:
- Enlarged RBCs, some with fine brownish granules
- > 15 parasites in some cells
- Ameboid structures
- Schuffner's dots
A. Plasmodium ovale
B. Plasmodium malariae
C. Plasmodium falciparum
D. Plasmodium vivax
Question 5
Transport media are required for throat culture swabs but not for rapid strep tests.
A. True
B. False
Solutions:
| Question 1 Answer: C | Question 2 Answer: A,D | Question 3 Answer: D | Question 4 Answer: D | Question 5 Answer: A |







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